Composite

Part:BBa_K2933233:Design

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-15)


RBS a+Linker g+GST+Linker e+JOHN-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 802
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 802
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113
    Illegal SapI.rc site found at 1326


Design Notes

This part is codon optimized in E. coli.


Source

NCBI

References